Basic information

Product Name: Recombinant Streptococcus Protein G (r-SPG)

Packing Details: 1 mg, 10 mg, 100 mg, 500 mg

Formulation: Lyophilized from 5 mM PB, pH 7.4

Mol. Wt.: 28 kDa

Resources: Escherichia coli (E. coli)

Purity: ≥90% by SDS-PAGE analysis

Storage Condition: -20oC

Storage Duration: 3 years

Description:

Protein G is a bacterial cell wall protein expressing at the cell surface of some group C and group G Streptococcal strains that binds the Fc region of immunoglobulin G (IgG) with high affinity. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to IgGs from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G shows a broader range of binding to IgG subclasses than does staphylococcal protein A. Some of the most important application areas for protein G are the isolation and purification or the removal of IgG from serum, the purification of monoclonal antibodies, and the isolation of immune complexes. Protein G conjugates is commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites, tissue culture supernatants and other biological fluids.

In addition to the Fc receptor, intact protein G has membrane spanning regions as well as specific binding sites for albumin and for the Fab region of immunoglobins. The albumin and cell surface binding domains have been eliminated from recombinant protein G to ensure the maximum specific IgG binding capacity.

Precautions:
1) Dissolve and place on ice bath and use as soon as possible;
2) If it can not be used in one time, please prepare the mother liquor before use and pack it into small size (guaranteed the amount of one test) and store it at -65 °C to avoid repeated freezing and thawing affecting protein activity.
3) Avoid the use of strong acids, strong bases, strong oxidants, high concentrations of organic solvents and other reagents that cause protein denaturation;
4) This product is limited to scientific research.