Recombinant Strep-Tactin with Cysteine r-STc

Introduction

Item Name:Recombinant Strep-Tactin with Cysteine, r-STc

Product form:lyophilized powder. The solution before lyophilization is 5 mM PB(2.5 mM Na₂HPO₄,2.5 mM NaH₂PO₄, pH 6.8)solution

Purity:≥95% (SDS-PAGE)

Molecular weight:Subunit monomer 13.4 kDa; tetramer 53.6 kDa

Sources:Escherichia coli (E. coli)

Isoelectric point:8.12

A_280nm(1 mg/mL):3.13

Theoretical activity:18.2 U/mg protein

Detection activity:≥15 U/mg protein

Specification:1 mg, 10 mg, 100 mg, 500 mg or special order

Storage conditions:Please store -20℃ for long term storage

Validity:3 years

Description:

Strep-Tactin is a mutant of Streptavidin that specifically binds to the Strep II tag. The Strep II tag is a small 8-amino acid tag (WSHPQFEK). Due to its small size, it is only about 1 kDa. It generally does not affect the structure and function of the protein after fusion. It is often used for the detection and purification of fusion proteins. Compared with streptavidin, Strep-Tactin has at least 10 times more affinity for the Strep II tag, and can bind and dissociate with Strep II fusion protein under mild conditions, and can bond to the medium. Good separation and purification. In order to be able to bind to the medium in a targeted manner, this product has a cysteine added to the C-terminus of the amino acid sequence, which can be covalently bonded to the medium via a thiol group.

Notes:

It is recommended that the product is reconstituted with sterile water into a final concentration of

0.5 mg/ml. Store the reconstituted product in aliquots at -20°C. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes.

The use of strong acids and alkalis, strong oxidants, and high concentrations of organic solvents should be avoided to protect the product from denaturation.

Packaging Details:
1mg,10mg, 100mg,500mg

Related products

Shipping: 

1) Door to Door Service by DHL/FEDEX/EMS/TNT for package under 45kgs, about 3~7 days delivery. 

2) Port to Port Service by Air for package more than 45kgs, about 3~7 days delivery.

3) Port to Port Service by Sea for package more than 500kgs, about 7~45 days delivery.

Company Information:

Wuhan W&Z Biotech Co,.Ltd is a high-tech enterprise which focus on the R&D, production, sales and service of biomedical and large health field. It has strong scientific research strength, modern management system and high-quality marketing team.

W&Z, located in Wuhan, the national key science and education base, cooperates with Wuhan University, Huazhong University of Science and Technology and other well-known universities, forming a good R&D and production team. in our R & D team, there are five experts in fields.

W&Z focus on provide CRO and CMO services for customers on one-stop intermediates, APIs and green technology. We have established modern R&d laboratories in Wuhan Optical Valley, have two partner factories in Shandong and Sichuan. Besides supplying standard products with mature technology, we also do customize under customer`s personalized needs.

We will uphold the quality first, service-oriented, integrity-based purpose, committed to provide more and more, better and better services to the global customers.

If you have any further questions or need a sample,Please do not hesitate to contact us!